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The HBsAg ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory test used to detect the presence of Hepatitis B surface antigen (HBsAg) in a person’s blood, indicating an active Hepatitis B virus (HBV) infection. This highly sensitive and specific test involves coating a plate with HBsAg-specific antibodies, allowing any HBsAg present in the sample to bind. A subsequent detection process produces a measurable signal, usually in the form of a color change. Results typically take several hours to a few days. A positive HBsAg ELISA result confirms active HBV infection, which requires further evaluation and monitoring for appropriate management and treatment.